PURIFICATION OF DNA FRAGMENTS, PLASMID DNA, AND RNA USING AEROSIL A-300 AND GF F (GF/C) FILTERS/

Plasmid DNA, native RNA, and DNA fragments were purified from agarose gels using aerosil A-300 and glass filters GF/F (GF/C). In the presenc e of guanidine thiocyanate, the latter selectively bind nucleic acids, while proteins, polysaccharides, salts, and agarose can be washed off . DNA and RNA preparations thus obtained can be used as substrates dir ectly: DNA can be digested with restriction endonucleases, ligated, se quenced, RNA can be used as a template for RT-PCR assay.