ELECTROFUSION OF MUCOR-CIRCINELLOIDES PROTOPLASTS

Authors
SOMOGYVARI F VAGVOLGYI C PAPP T
Citation
F. Somogyvari et al., ELECTROFUSION OF MUCOR-CIRCINELLOIDES PROTOPLASTS, Biotechnology techniques, 10(8), 1996, pp. 607-610
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
8
Year of publication
1996
Pages
607 - 610
Database
ISI
SICI code
0951-208X(1996)10:8<607:EOMP>2.0.ZU;2-1
Abstract
Electrical parameters were optimized for induction of the fusion of pr otoplasts formed from two auxotrophic (leu(-), ade(-)) Mucor circinell oides strains. These proved to be a pulse voltage of 66 V, a pulse dur ation of 66 Cls and six pulses at intervals of 1.0 s. Nutritionally co mplementing colonies were obtained at an average frequency up to 12.4% , which is substantially higher than achieved earlier with the PEG-Ca2 + method.