Av. Vakurov et al., NONCOVALENT ENZYME-POLYELECTROLYTE COMPLEXES AS SELF-BUFFERED CATALYSTS FOR USE IN LOW-WATER ORGANIC MEDIA, Biotechnology techniques, 10(8), 1996, pp. 621-624
When free chymotrypsin is used to catalyse hydrolysis of N-acetyl tyro
sine ethyl ester in 90% acetonitrile, the reaction rate soon falls bec
ause of the accumulation of the acidic product. If the enzyme is used
in the form of a suspended complex with polyacrylic acid, the polyelec
trolyte acts as an acid-base buffer to permit extended reaction.