NONCOVALENT ENZYME-POLYELECTROLYTE COMPLEXES AS SELF-BUFFERED CATALYSTS FOR USE IN LOW-WATER ORGANIC MEDIA

Authors
VAKUROV AV GLADILIN AK PARTRIDGE J MOZHAEV VV LEVASHOV AV HALLING PJ
Citation
Av. Vakurov et al., NONCOVALENT ENZYME-POLYELECTROLYTE COMPLEXES AS SELF-BUFFERED CATALYSTS FOR USE IN LOW-WATER ORGANIC MEDIA, Biotechnology techniques, 10(8), 1996, pp. 621-624
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
8
Year of publication
1996
Pages
621 - 624
Database
ISI
SICI code
0951-208X(1996)10:8<621:NECASC>2.0.ZU;2-D
Abstract
When free chymotrypsin is used to catalyse hydrolysis of N-acetyl tyro sine ethyl ester in 90% acetonitrile, the reaction rate soon falls bec ause of the accumulation of the acidic product. If the enzyme is used in the form of a suspended complex with polyacrylic acid, the polyelec trolyte acts as an acid-base buffer to permit extended reaction.