LACCASE VARIATION DURING DYE DECOLORIZATION IN A 200-L PACKED-BED BIOREACTOR

The white-rot fungus, Pycnoporus cinnabarinus, was grown in a 200 L pa cked-bed bioreactor. The dye, Remazol Brilliant Blue R, was added to t he reactor and was rapidly decolourised. The phenoloxidase, laccase, c atalysed this process. Laccases isolated from different days in the bi oreactor cycle indicated variations in charge to size characteristics. Purified laccase from different days was isoelectrically focused and showed variations in intensities and numbers of bands whilst catalytic activity was retained.