ADSORPTION OF PHAGE-LAMBDA TO SALMONELLA-TYPHIMURIUM LAMB(-MEMBRANE()REQUIRES THE PRESENCE OF LIPOPOLYSACCHARIDE IN THE OUTER)

Authors
ELMZIBRI M BEUDOT C DEMEO M LAGET M GUIRAUD H DUMENIL G
Citation
M. Elmzibri et al., ADSORPTION OF PHAGE-LAMBDA TO SALMONELLA-TYPHIMURIUM LAMB(-MEMBRANE()REQUIRES THE PRESENCE OF LIPOPOLYSACCHARIDE IN THE OUTER), Biotechnology letters, 18(8), 1996, pp. 933-936
Citations number
17
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
8
Year of publication
1996
Pages
933 - 936
Database
ISI
SICI code
0141-5492(1996)18:8<933:AOPTSL>2.0.ZU;2-Y
Abstract
Two S. typhimurium strains TA1534 (rfa(+)) and TA1538 (rfaE) were tran sformed with the lamB expression plasmid pAMH70. Transposition events with lambda placMu55 hybrid phage were successful only with TA1534/pAM H70 strain. Using SDS-PAGE, the LamB protein was present in the total cell proteins but not in the outer membrane proteins of the TA1538/pAM H70 strain. The LamB protein must linked to the LPS of the outer membr ane to allow adsorption of lambda phage in S. typhimurium.