Among twelve strains of Clostridium thermocellum isolated from faecal
droppings of various herbivorous animals and birds, three of the strai
ns, SS21, SS22 and SS19, produced 0.37, 0.33 and 0.32 g of ethanol per
g of the substrate consumed and had ethanol to acetate ratios of 2.21
, 2.45 and 1.72 respectively. These are the highest substrate conversi
on yields of ethanol amongst the wild strains of C. thermocellum repor
ted so far. The optimum temperature and pH for growth and ethanol prod
uction were 60 degrees C and 7.5, respectively.