IN-VITRO PROPAGATION OF RAUVOLFIA-SERPENTINA THROUGH TISSUE-CULTURE

Multiple shoots were induced from nodal segments and shoot apices of R auvolfia serpentine and MS medium containing 1.0 mg/l BA and 0.1 mg/l NAA was found to give the best shoot proliferation rate. Callus formed at cut bases of the explants which produced shoots when subcultured o n media containing low concentration of BA (0.5 or 0.1 mg/l) and NAA ( 0.1 mg/l). The in vitro proliferated shoots were rooted and later tran sferred to the soil.