QUANTITATIVE CHROMATOGRAPHIC DETERMINATION OF VIRGINIAMYCIN ANTIBIOTICS

Analytical methods have been developed for determination of the indivi dual components of Virginiamycin antibiotics during the process of mic robiological synthesis. The reference substances (virginiamycins M1, M 2, and S1-S5) were extracted from the fermentation broth and purified by preparative HPLC. Analysis of virginiamycin was performed by HPTLC and HPLC. Coefficients of variation between the two techniques were ca 5 %, and there was good correlation (r = 0.993). Experimental results demonstrate a relationship between component composition of the antib iotics and the genetic strain of their producers.