T. Suzuki et al., QUANTITATIVE-DETERMINATION OF THE MAIN HYDROXYANTHRAQUINONES IN SOILSBY TLC WITH 2-STAGE DEVELOPMENT, JPC. Journal of planar chromatography, modern TLC, 9(1), 1996, pp. 48-51
The separation and quantitation of the five main hydroxyanthraquinones
in soils has been performed by TLC with two development steps and den
sitometric scanning. The five hydroxyanthraquinones were completely re
solved both from each other and from other accompanying compounds and
were quantified hy densitometry in reflectance mode at a sample wavele
ngth of 445 nm and a reference wavelength of 540 nm. The detection lim
it of the method was in the lower nanogram range (5-10 ng) and the pea
k area response was linearly dependent on quantity in the range 1-300
ng.