QUANTITATIVE-DETERMINATION OF THE MAIN HYDROXYANTHRAQUINONES IN SOILSBY TLC WITH 2-STAGE DEVELOPMENT

The separation and quantitation of the five main hydroxyanthraquinones in soils has been performed by TLC with two development steps and den sitometric scanning. The five hydroxyanthraquinones were completely re solved both from each other and from other accompanying compounds and were quantified hy densitometry in reflectance mode at a sample wavele ngth of 445 nm and a reference wavelength of 540 nm. The detection lim it of the method was in the lower nanogram range (5-10 ng) and the pea k area response was linearly dependent on quantity in the range 1-300 ng.