ANALYSIS OF HYDROXY-PAH BY THIN-LAYER CHROMATOGRAPHY AND OPLC

Reversed phase planar chromatographic separations of hydroxy derivativ es of polycyclic aromatic hydrocarbons have been performed with aceton itrile - water and methanol - water as mobile phases. Separations perf ormed both in a DS sandwich chamber (TLC) and in an overpressured cham ber (OPLC) produced in Poland were compared after visualization of the spots both in UV light (wavelengths 254 and 365 nm) and by means of r eaction with fast blue B salt. Both techniques gave good results for t he separation of the compounds; forced how techniques such as OPLC may be suitable for routine analysis of the compounds. Analysis by GC-MS has confirmed the presence of 1-hydroxypyrene and 9-hydroxyphenanthren e in extracts from TLC plates used to separate the dichloromethane fra ction isolated from airborne particulate matter sampled in the Upper S ilesia region.