EXOGENOUS ESTROGEN ACTS DIFFERENTLY ON PRODUCTION OF ESTROGEN-RECEPTOR IN THE PREOPTIC AREA AND THE MEDIOBASAL HYPOTHALAMIC NUCLEI IN THE NEWBORN RAT

Expression of the estrogen receptor (ER) in the preoptic area (POA) an d the mediobasal hypothalamus (MBH) in newborn female rats was studied by immunohistochemistry (IHC) and in situ hybridization histochemistr y (ISHH). The number of ER immunoreactive (ER-IR) cells decreased and expression of ER mRNA was suppressed in the arcuate (ARH) and the vent romedial (VMH) hypothalamic nuclei by daily injections of estradiol be nzoate (EB) for ten consecutive days. In contrast, in the POA, express ion of ER mRNA was not suppressed by EB treatment, while the ER immuno reactivity and the number of ER-IR cells was decreased by EB treatment . Results of quantification of ER mRNA by reverse transcription-polyme rase chain reaction correlated well with results from ISHH: that is: E R mRNA expression decreased in the MBH but not in the POA. Thus, estro gen affects ER gene expression differently in these two brain regions.