Retrograde and anterograde neuronal tracers (HRP, biocytin, biotinylat
ed dextran-amine) were used to study the organisation of trigeminocere
bellar and trigemino-olivary connections, focusing on the connectivity
between trigeminal nuclear regions and the sagittal zones of the rat
cerebellar cortex. Trigeminocerebellar projections were bilateral, but
mostly ipsilateral. Direct trigeminocerebellar fibres originated most
ly in the principal trigeminal nucleus (VP) and pars oralis (Vo), pars
interpolaris (Vi), and to a lesser extent in pars caudalis (Vc) of th
e spinal trigeminal nucleus. Consistent projections were found from th
e Vc to cerebellar lobules IX and X. The trigeminal fibres terminated
in the cerebellum in an organised fashion. The ventral part of the VP,
Vo and Vi projected to the medial A zone and the C3 and D2 subzones,
whereas the dorsal part of the nuclei projected to the lateral A zone
and the C2, D0 and D1 subzones. In lobules IX and X, the organisation
was different. The medial half of the VP, Vo, Vi and Vc projected to t
he lateral aspects of these lobules whereas their lateral part project
ed to their medial aspects. Trigeminal projections to the deep cerebel
lar nuclei were also present. Projections to a given sagittal zone con
comitantly reached its corresponding nuclear target. Trigemino-olivary
projections were principally contralateral. The Vo, Vi and Vc project
ed to the rostromedial dorsal accessory olive, the adjacent dorsal lea
f and the dorsomedial part of the ventral leaf of the principal olive,
which are known to project subzones C3, D0 and D1 of the rat cerebell
ar cortex.