THE ROLE OF ENDOGENOUS BRADYKININ IN BLOOD-PRESSURE HOMEOSTASIS IN SPONTANEOUSLY HYPERTENSIVE RATS

1 The role of endogenous bradykinin in mean arterial blood pressure (B P) homeostasis was studied in spontaneously hypertensive (SHR) and nor motensive (WKY) rats by the use of a bradykinin B-2-receptor antagonis t (BKant; Hoe 140, 11.6 mu g kg(-1)) and converting enzyme (kininase I I) inhibitor (captopril, 10 mg). To obtain a response to captopril tha t was induced through inhibition of kinin-degradation only and not thr ough inhibition of angiotensin II-formation, the studies were performe d on binephrectomized male rats to eliminate the renin-angiotensin sys tem. 2 The role of the nitric oxide (NO) and the adrenergic systems we re evaluated by the use of NO-synthase inhibitor (L-NAME, 0.3 g kg(-1) ) and phentolamine (2 mg kg(-1)), respectively. 3 The rats were anaest hetized and pretreated with two injections of vehicle (PBS) or drugs s paced 5 min apart: PBS + PBS; BKant + PBS; PBS + L-NAME; BKant + L-NAM E; or phentolamine + L-NAME. All rats were given captopril 15 min late r. Time-control groups were treated with L-NAME but not captopril. 4 I n WKY rats, captopril did not significantly alter BP in any of the gro ups. In the SHR-PBS + PBS group, on the other hand, captopril induced an immediate fall in BP (Delta BP = -23 +/- 4 mmHg, P<0.0017) which wa s completely blocked by BKant (Delta BP = 2 +/- 2 mmHg) (P<0.0011). L- NAME did not significantly alter the immediate hypotensive response to captopril but disclosed a later hypertensive reaction. In L-NAME + BK ant-treated rats, both the hypotensive response and the late hypertens ion was abolished. In rats treated with phentolamine + L-NAME, the imm ediate fall in BP was not different from the controls whereas the late hypertension was absent. 5 BKant itself had no effect on basal BP in either WKY or SHR even when a 10 times higher dose was tested in a sep arate set of experiments. This was true also for conscious, nonnephrec tomized SHR rats. 6 It was concluded that endogenous production of bra dykinin was demonstrable through kininase II-inhibition in hypertensiv e but not in normotensive rats. However, this endogenous bradykinin di d not play a role in basal BP homeostasis. The captopril-induced hypot ension depended on kinin but, under the present conditions, not on NO as a mediator. The fall in BP induced a compensatory adrenergic hypert ensive response which was revealed when the continuous NO-synthesis wa s blocked by L-NAME.