B. Popping et al., THE PISUM-SATIVUM MAP KINASE HOMOLOG (PSMAPK) RESCUES THE SACCHAROMYCES-CEREVISIAE HOG1 DELETION MUTANT UNDER CONDITIONS OF HIGH OSMOTIC-STRESS, Plant molecular biology, 31(2), 1996, pp. 355-363
Previous analysis of the MAP kinase homologue from Pisum sativum (PsMA
PK) revealed a potential MAP kinase motif homologous to that found in
eukaryotic cdc2 kinases. Sequence comparison showed a 47% identity on
amino acid sequence basis to the Saccharomyces cerevisiae Hog1p MAP ki
nase involved in the osmoregulatory pathway. Under conditions of salt-
stress aberrant morphology of a hog1 deletion mutant was completely re
stored and growth was partially restored by expression of the PsMAPK.
This shows that PsMAPK is functionally active as a MAP kinase in S. ce
revisiae. Comparison of PsMAPK with other kinases involved in osmosens
itivity, showed a high degree of homology and implicates a possible ro
le for PsMAPK in a P. sativum osmosensing signal transduction pathway.