DIRECT DETERMINATION OF IRON IN FERRITIN USING THE POLAROGRAPHIC CATALYTIC CURRENT

A new method for the determination of iron in ferritin using the polar ographic catalytic current has been developed. Iron is released in NaO H and triethanolamine (TEA) solution and subsequently determined based on the polarographic catalytic wave in the presence of bromate with t he peak potential of -1.0 V (vs.SCE). The 2.5-derivative peak height o f iron is linearly proportional to its concentration in the range of 8 .0 x 10(-8)-1.0 x 10(-6) g/ml with a correlation coefficient of 0.9991 . The method has been applied to the determination of iron in human fe tal liver and horse-spleen ferritin and the recovery is 95-112%.