SYNTHESIS OF HYALURONIC-ACID BY HUMAN PERITONEAL MESOTHELIAL CELLS - EFFECT OF CYTOKINES AND DIALYSATE

Objective: To assess effects of the inflammatory cytokines (IL-1-beta, TNF-alpha, TGF-beta 1) and dialysate effluent on synthesis of hyaluro nic acid by human peritoneal mesothelial cells (HMC) in in vitro cultu re. Methods: Dialysate effluent was collected after the overnight dwel l of Dianeal 1.5% from patients during CAPD training. HMC were obtaine d from omentum from nonuremic donors or were harvested from the dialys ate effluent from CAPD patients. Synthesis of hyaluronic acid was stud ied on monolayers of HMC, which were deprived of serum 48 hours prior to experiment. Effects of cytokines were tested in a medium with low s erum concentration (0.1%) or in medium mixed (1:1 v/v) with the autolo gous dialysate. Hyaluronic acid level in medium was measured with radi oimmunoassay. Results: Cytokines enhanced synthesis of hyaluronic acid by HMC, and the strongest effect was induced by IL-l. Effluent dialys ate stimulates synthesis of hyaluronic acid stronger than 10% FCS. Eff luent dialysate and IL-l synergistically enhance synthesis of hyaluron ic acid by HMC. Conclusion: Effluent dialysate from CAPD patients stim ulates production of hyaluronic acid by HMC and acts synergistically w ith cytokines.