ISOLATION OF MINOR LUPIN ALKALOIDS .1. A SIMPLE PROCEDURE FOR THE ISOLATION OF ANGUSTIFOLINE FROM LUPINUS-ANGUSTIFOLIUS (CV FEST) SEEDS, WITH APPLICATION TO OTHER LUPIN ALKALOIDS

The lupins, especially Lupinus angustifolius, are an important source of high protein food, which also benefit the soil by nitrogen fixation during growth. Angustifoline, a minor alkaloid of L. angustifolius, h as been isolated by derivitization as the trichloroacetate followed by base hydrolysis. Other derivatives, such as the acetate, could not be satisfactorily hydrolyzed to the base alkaloid without extensive deco mposition. A second method of isolation was developed using solvent ex traction from pH-controlled solutions, from pH 7 to 13, yielding relat ively pure crystalline angustifoline. This pH-controlled procedure has been successfully applied to the isolation of epilupinine and multifl orine from the mixed alkaloids of Lupinus atlanticus.