METHIONINE CONTENT OF THE POLYPEPTIDES OF METHIONINE-RICH PROTEIN FROM PEANUT

Authors
SATHANOORI RS BASHA SM
Citation
Rs. Sathanoori et Sm. Basha, METHIONINE CONTENT OF THE POLYPEPTIDES OF METHIONINE-RICH PROTEIN FROM PEANUT, Journal of agricultural and food chemistry, 44(8), 1996, pp. 2134-2136
Citations number
11
Categorie Soggetti
Food Science & Tenology",Agriculture,"Chemistry Applied
Journal title
Journal of agricultural and food chemistry → ACNP
ISSN journal
00218561
Volume
44
Issue
8
Year of publication
1996
Pages
2134 - 2136
Database
ISI
SICI code
0021-8561(1996)44:8<2134:MCOTPO>2.0.ZU;2-N
Abstract
The six subunits of the methionine-rich protein (MRP) from peanut seed were separated using two-dimensional gel electrophoresis followed by western blotting of MRP. The PVDF membrane blots containing the MRP su bunits were acid hydrolyzed and analyzed for amino acid composition. T he results showed that of the six MRP subunits the low molecular weigh t (15 500) polypeptides, MRP-5 and MRP-6, contained the highest amount s (3.8% and 4.6%, respectively) of methionine, while the methionine co ntent of the other four subunits was between 1.7% and 1.9%.