HIGH-PERFORMANCE CAPILLARY ELECTROPHORETIC METHODS FOR THE DETERMINATION OF ANTIBIOTIC FUNGICIDE KASUGAMYCIN IN FORMULATED PRODUCTS

A high-performance capillary electrophoretic (HPCE) method with a UV-v is detector was used to determine kasugamycin content in commercial pr oducts. The results indicated that this method was capable of analyzin g the kasugamycin content in formulated products with an instrument de tection Limit of 0.44 mu g/mL and a method detection limit of 0.51 mu g/mL. Recoveries of kasugamycin in formulated products were in the reg ion of 99.9-103.2%. Relative standard deviation (RSD) values of HPCE d etermination of kasugamycin in formulated products ranged from 0.57 to 2.22%. All products collected from markets contained kasugamycin and were real products. High recovery, low detection limit, and low RSD va lues confirmed that the HPCE method is a sensitive and selective metho d. Thus the present official bioassay method could be replaced by the HPCE method.