EFFECT OF TEMPERATURE AND GENOTYPE ON THE CRUDE GLYCININ FRACTION (11S) OF SOYBEAN AND ITS ANALYSIS BY NEAR-INFRARED REFLECTANCE SPECTROSCOPY (NEAR-IRS)

The crude glycinin fraction (11S) of soybean [Glycine max (L.) Merr.] protein has desirable quality characteristics, The objectives of this soybean study were the following: (1) to develop a near-infrared refle ctance spectroscopy (near-IRS) equation for screening of the 11S fract ion; (2) to determine relationships between the 11S fraction, crude pr otein, and oil concentration; and (3) to study the effect of growing t emperature and genotype on this fraction. Three near-IRS 11S equations were developed, with the best equation having a moderate standard err or of calibration (7.7 g/kg) and coefficient of determination (R(2) = 0.84). A positive correlation was observed between the 11S protein sub unit and crude protein concentration (r = 0.69, P < 0.01), and a negat ive correlation was found between the 11S protein subunit and oil conc entration (r = -0.67, P < 0.01), based on 319 genotypes. Higher crude protein and 11S protein subunit concentrations were associated with hi gher growing temperatures, and higher oil concentration was associated with lower growing temperatures, based on 12 genotypes.