Ma. Coimbra et al., EFFECT OF PROCESSING ON CELL-WALL POLYSACCHARIDES OF GREEN TABLE OLIVES, Journal of agricultural and food chemistry, 44(8), 1996, pp. 2394-2401
Cell wall material (CWM) was prepared from green olive pulp processed
according to the ''Sevillian Style'', Polymers were solubilized from t
he CWM by the sequential extraction with trans-1,2-cyclohexanediamine-
N,N,N',N'-tetraacetic acid, sodium salt (CDTA); Na2CO3; 1 M KOH; 4 M K
OH; and 4 M KOH + berate to leave a cellulosic residue (CR). The polym
ers from the various extracts were fractionated and selected fractions
were subjected to methylation analysis. This study showed that the so
ftening of the olive pulp with the processing was due to a partial deg
radation of the pectic polysaccharides. Part of the degraded polymers
were solubilized during the preparation of the CWM and the majority we
re solubilized after CDTA treatment. The CWM also showed coprecipitati
on with intracellular proteins and phenolic compounds that originated
a material that was insoluble in aqueous solutions. The hemicellulosic
polymers were not significantly affected by the processing.