ONE-POT ENZYMATIC-SYNTHESIS OF THE GAL-ALPHA-1-]3CAL-BETA-1-]4GLCNAC SEQUENCE WITH IN-SITU UDP-GAL REGENERATION

Authors
HOKKE CH ZERVOSEN A ELLING L JOZIASSE DH VANDENEIJNDEN DH
Citation
Ch. Hokke et al., ONE-POT ENZYMATIC-SYNTHESIS OF THE GAL-ALPHA-1-]3CAL-BETA-1-]4GLCNAC SEQUENCE WITH IN-SITU UDP-GAL REGENERATION, Glycoconjugate journal, 13(4), 1996, pp. 687-692
Citations number
25
Categorie Soggetti
Biology
Journal title
Glycoconjugate journal → ACNP
ISSN journal
02820080
Volume
13
Issue
4
Year of publication
1996
Pages
687 - 692
Database
ISI
SICI code
0282-0080(1996)13:4<687:OEOTGS>2.0.ZU;2-C
Abstract
The trisaccharide Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2) (8)COOCH3 was enzymatically synthesized, with in situ UDP-Gal regenera tion. By combination in one pot of only four enzymes, namely, sucrose synthase, UDP-Glc 4'-epimerase, UDP-Gal:GlcNAc beta 4-galactosyltransf erase and UDP-Gal:Gal beta 1-->4GlcNAc alpha 3-galactosyltransferase, Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)(8)COOCH3 was form ed in a 2.2 mu mol ml(-1) yield starting from the acceptor GlcNAc beta 1-->O-(CH2)(8)COOCH3. This is an efficient and convenient method for the synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc epitope which plays an important role in various biological and immunological proce sses.