J. Pintor et al., DIADENOSINE POLYPHOSPHATE-ACTIVATED INWARD AND OUTWARD CURRENTS IN FOLLICULAR OOCYTES OF XENOPUS-LAEVIS, Life sciences, 59(12), 1996, pp. 179-184
Citations number
14
Categorie Soggetti
Biology,"Medicine, Research & Experimental","Pharmacology & Pharmacy
Ionic currents evoked by alpha,omega-adenine dinucleotides (Ap(X)A; X=
2-6) in follicular oocytes of Xenopus laevis were studied under voltag
e-clamp conditions. Dinucleotides evoked inward and outward currents i
n Xenopus oocytes by activating native P1 and P2 purinoceptors known t
o be present on the follicle cell monolayer enveloping oocytes. Inward
currents were mediated by a suramin-sensitive P2 purinoceptor which s
howed an agonist potency order (at 10 mu M): Ap(4)A>ATP>Ap(3)A>>Ap(5)A
, while Ap(2)A and Ap(6)A were inactive. Outward currents were mediate
d by a novel theophylline-sensitive P1 purinoceptor which showed an ag
onist potency order (at 10 mu M): Ap(2)A>ATP>>Ap(4)A=Ap(5)A=Ap(6)A>Ap(
3)A. Chromatographic analysis confirmed ectonucleotidase activity at t
he follicle cell layer of oocytes but at a very low rate of dinucleoti
de cleavage, indicating that currents evoked by dinucleotides resulted
fi om a direct activation of oocyte P1 and P2 purinoceptors and not t
hrough their breakdown to ATP, ADP and AMP. There was no evidence for
specific receptors (i.e., P4 purinoceptors) for diadenosine polyphosph
ates in Xenopus oocytes.