SUPPRESSION OF TUMOUROGENICITY AND OVEREXPRESSION OF CELL-ADHESION MOLECULES IN AK-5 CELLS TRANSFECTED WITH WILD-TYPE P53 GENE

We have previously shown overexpression of p53 and rearrangement of th e p53 gene in AK-5 tumour. In order to study the role of p53 in AK-5 t umourogenicity, we introduced wild-type p53 in AK-5 cells. We have sho wn suppression of tumourogenicity in AK-5 cells after transfection wit h wt p53, In one of the transfected clones 3B4, there was complete los s of tumour forming ability, Clone 3B4 also shelved cellular aggregati on which correlated well with the higher expression of cell adhesion m olecules like fibronectin and hyaluronectin. These observations demons trate tumour suppression in AK-5 after introduction of wt p53.