QUALITATIVE AND QUANTITATIVE DETECTION OF AFLATOXIN B-1 IN POULTRY SERA BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY

Citation
Cps. Sekhon et al., QUALITATIVE AND QUANTITATIVE DETECTION OF AFLATOXIN B-1 IN POULTRY SERA BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY, Journal of Biosciences, 21(4), 1996, pp. 471-476
Citations number
14
Categorie Soggetti
Biology
Journal title
ISSN journal
02505991
Volume
21
Issue
4
Year of publication
1996
Pages
471 - 476
Database
ISI
SICI code
0250-5991(1996)21:4<471:QAQDOA>2.0.ZU;2-V
Abstract
An indirect competitive inhibition type enzyme-linked immunosorbent as say (ELISA) has been developed for the detection of aflatoxin B-1 in p oultry sera. Preincubation of aflatoxin B-1 samples with the antibody prior to competition yielded better results in terms of higher sensiti vity. After competition, amount of antibody bound to solid phase was m easured by incubation with anti-rabbit immunoglobulins coupled with ho rse raddish peroxidase, Intensity of colour decreased as the amount of free aflatoxin B-1 increased. Final detection of aflatoxin B-1 was ma de by (i) visual comparison with standard aflatoxin B-1, using dot-ELI SA (qualitative) and (ii) by plate-ELISA, where optical density was me asured at 492 nm (quantitative). Plate-ELISA was more sensitive than d ot-ELISA, with sensitivity limits being 100 fg and 1 pg per 10 mu l, r espectively. However, due to ease and speed of performance, dot-ELISA has greater potential as a test for the diagnosis of mycotoxicosis at the field level.