REVERSION OF MULTIDRUG-RESISTANCE USING NANOPARTICLES IN-VITRO - INFLUENCE OF THE NATURE OF THE POLYMER

Previous studies have already shown that polyalkylcyanoacrylate nanopa rticles were able to overcome multidrug resistance in vitro. The study described in this paper investigated other types of nanoparticles, ma de of poly (lactic acid) PLA, poly (lactic-co-glycolic acid) PLGA and alginate, in order to determine if overcoming of resistance could be a chieved with any nanoparticles independently of the nature of the poly mer. In a first step, PLA and PLGA nanoparticles loaded with doxorubic in have been formulated, and the cytotoxicity of each preparation has been evaluated against P388 sensitive and resistant cells. Only polyal kylcyanoacrylate nanoparticles were effective: doxorubicin release exp eriments allowed to incriminate too rapid doxorubicin release in the c ase of PLA, PLGA and alginate nanoparticles to explain their lack of e fficacy to overcome MDR.