CHROMOSOME-11 ALTERATIONS IN NON-SMALL-CELL LUNG CARCINOMAS IN HONG-KONG

We examined 60 non-small-cell lung cancer (NSCLC) patients for evidenc e of genetic alterations on chromosome 11 with nine polymorphic marker s by Southern blot and microsatellite marker analysis. These analyses detected genetic alterations at both the 11p and 11q arms. At the 11p1 5 Ha-ras locus, the loss of heterozygosity (LOH) occurred in three out of 11 (27.3%) of the informative cases; at the 11p11-q12 D11S149 locu s, the LOH occurred in two out of nine (22.2%) of the informative case s; and at the 11q13 INT-2 locus, the LOH occurred in four out of 18 (2 2.2%) of the informative cases. Microsatellite markers in the 11q12-q1 3 region revealed genetic alterations for PYGM in eight out of 54 (14. 8%) of the specimens studied and 10 out of 55 (18.2%) of the specimens for the INT-2 marker. The data suggest genetic alterations occur in s ome of the lung cancer patients in both the 11p and 11q regions.