FOOD-DEPRIVATION AND THE FACILITATORY EFFECTS OF ESTROGEN IN FEMALE HAMSTERS - THE LH SURGE AND LOCOMOTOR-ACTIVITY

Two experiments investigated short-term food deprivation effects on ne uroendocrine processes influenced by estrogen. These studies were prom pted by prior work indicating that food deprivation increased the numb er of immunocytochemically identified cells containing estradiol recep tors in the medial preoptic area of ovariectomized female hamsters. Pr esumably, this is one way that changes in metabolic fuel availability might alter the responsiveness of one or more systems to estradiol. Th e purpose of this study was to investigate two effects of estradiol th at might be affected by food deprivation; these were 1) the positive f eedback effects of estradiol on the luteinizing hormone (LH) surge, an d 2) the facilitating effects of estradiol on locomotor activity. In E xperiment 1, ovariectomized hamsters were administered estradiol, befo re or after 48 h of food deprivation. Two days after hormone treatment , blood was obtained by cardiac puncture, once in the morning (1100 h) and twice during the afternoon (1600-1800 h). These times were chosen to best characterize the magnitude of the LH surge. Food deprivation enhanced the amplitude of the LH surge in response to estradiol when t his treatment preceded, but not when it followed, the administration o f estradiol. However, there was variability in the dose of estradiol a t which this effect of food deprivation occurred. In Experiment 2, the locomotor (running wheel) activity of two groups of gonadally intact female hamsters was quantified; one group was tested during the early (days 1 + 2; low estradiol) part of the estrous cycle, and the other g roup was tested during the late (days 3 + 4; high estradiol) part of t he estrous cycle. In both groups, testing was performed first under ad lib feeding conditions and again during 48 h of food deprivation. On average, the days 3 + 4 group was more active than the days 1 + 2 grou p, reflecting their differing levels of endogenous estradiol. Food dep rivation significantly increased locomotor activity, independently of the stage of the estrous cycle during which it was imposed. These resu lts are discussed in terms of the influence that altered estradiol rec eptor expression in the medial preoptic area might play in generating the effects we observed following short-term food deprivation.