EVALUATION OF REVERSED-PHASE COLUMNS FOR THE ANALYSIS OF DIPHENYLMETHANE DERIVATIVES

Citation
A. Martinsen et al., EVALUATION OF REVERSED-PHASE COLUMNS FOR THE ANALYSIS OF DIPHENYLMETHANE DERIVATIVES, Journal of liquid chromatography & related technologies, 19(16), 1996, pp. 2579-2592
Citations number
18
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
ISSN journal
10826076
Volume
19
Issue
16
Year of publication
1996
Pages
2579 - 2592
Database
ISI
SICI code
1082-6076(1996)19:16<2579:EORCFT>2.0.ZU;2-W
Abstract
Four reversed phases were tested in RPLC of newly synthesized diphenyl methane derivatives. Non-deactivated Kromasil C-8 and Beckman C-18 and deactivated Supelco LC-8-DB and Supelco LC-IX-DB reversed phases were evaluated. Column supplier, deactivation and the presence of amine mo difier (triethylamine, TEA) were used as variables and capacity factor s (k) and asymmetry factors (As) were measured. Two different mobile p hase systems were used: Acetonitrile: ammonium acetate (0.1 M) and met hanol: potassium dihydrogen phosphate (0.02 M). According to the resul ts of this comparative study, non-deactivated Kromasil C-8 and Beckman C-18 were found to be the most suitable phases for our applications e .g., synthesis purity and lipophilicity studies concerning antihistami ne-like compounds. For most of the compounds involved in this study, t he use of these phases requires TEA as an amine modifier. Addition of the amine also made it possible to use the deactivated phases, e.g., S upelco C-8 and C-18 columns. The RPLC method was used to evaluate the lipophilicity of some DPPE derivatives, compared to known antihistamin ergics.