A. Martinsen et al., EVALUATION OF REVERSED-PHASE COLUMNS FOR THE ANALYSIS OF DIPHENYLMETHANE DERIVATIVES, Journal of liquid chromatography & related technologies, 19(16), 1996, pp. 2579-2592
Citations number
18
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Four reversed phases were tested in RPLC of newly synthesized diphenyl
methane derivatives. Non-deactivated Kromasil C-8 and Beckman C-18 and
deactivated Supelco LC-8-DB and Supelco LC-IX-DB reversed phases were
evaluated. Column supplier, deactivation and the presence of amine mo
difier (triethylamine, TEA) were used as variables and capacity factor
s (k) and asymmetry factors (As) were measured. Two different mobile p
hase systems were used: Acetonitrile: ammonium acetate (0.1 M) and met
hanol: potassium dihydrogen phosphate (0.02 M). According to the resul
ts of this comparative study, non-deactivated Kromasil C-8 and Beckman
C-18 were found to be the most suitable phases for our applications e
.g., synthesis purity and lipophilicity studies concerning antihistami
ne-like compounds. For most of the compounds involved in this study, t
he use of these phases requires TEA as an amine modifier. Addition of
the amine also made it possible to use the deactivated phases, e.g., S
upelco C-8 and C-18 columns. The RPLC method was used to evaluate the
lipophilicity of some DPPE derivatives, compared to known antihistamin
ergics.