HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TEFLUBENZURON AND DIFLUBENZURON IN FISH-TISSUES OF FARMED FISH FOR RESIDUE STUDIES

A method for the determination of teflubenzuron and Diflubenzuron in f ish tissues by HPLC is presented. The samples were extracted with acet one - tetrahydrofuran. Employing traditional liquid-liquid extraction and using a Si and C-8 solid phase extraction column, a clean extract was obtained. The lower limit of quantification for teflubenzuron was 20 ng/g (mL) for muscle, liver and plasma and for diflubenzuron in mus cle and liver, the limit of quantifcation was 25 ng/g.