J. Chehimi et al., DIFFERENTIAL PRODUCTION OF INTERLEUKIN-10 DURING HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION, AIDS research and human retroviruses, 12(12), 1996, pp. 1141-1149
Interleukin 10, a product of T and B cells and monocytes, displays man
y Th2-like properties through inhibition of Th1 cell functions, Interl
eukin 10 is thought to play a major role in the immune dysfunction see
n in HIV-infected individuals, In this study, we evaluated in detail t
he production of IL-10 during HIV infection, Although the constitutive
production of IL-10 did not differ in PBMCs from healthy donors and H
IV-infected individuals, IL-10 was differentially produced in response
to polyclonal activators, The overall plasma IL-10 levels were simila
r in 32 controls and 67 patients at different stages of the disease an
d receiving different antiretroviral drugs. Hovever, patients with low
CD4 T cell count ( < 200/mm(3)) secreted approximately threefold more
IL-10 than did patients with high CD4 T cell count ( > 500/mm(3)), Co
mpetitive/quantitative PCR revealed similar levels of mRNA expression
in PBMCs from controls and HIV-infected individuals, In vitro HIV infe
ction rapidly and transiently induced IL-10 production in PBMCs and mo
nocytes, and the low level of endogenously secreted IL-10 failed to in
hibit HIV replication in acutely infected monocytes, On the other hand
, HIV infection of selected CD4(+) T cell clones generated in a Th1- o
r Th2-like environment, differentially upregulated IL-10 production, w
ith significantly higher production by Th2 clones, Together, our data
indicate that IL-10 production is more complex than previously thought
, and may depend on several factors such as producer cells, nature of
the stimuli, as well as viral isolates.