L. Garg et al., SOMATIC EMBRYOGENESIS AND REGENERATION OF TRIPLOID PLANTS IN ENDOSPERM CULTURES OF ACACIA-NILOTICA, Plant cell reports, 15(11), 1996, pp. 855-858
Immature endosperm of Acacia nilotica formed a nodular callus on MS me
dium supplemented with 2,4-D, BAP and CH. In the third passage on this
medium, in the dark, the callus differentiated somatic embryos. The e
mbryos germinated on MS only after 15 d pre-treatment on modified MS m
edium in which major salts were replaced by those of major salts of B-
5 medium and supplemented with glutamine, CH and CW. Triploid nature o
f the somatic embryos was confirmed by Feulgen cytophotometry.