THE ECONOMIC PRODUCTION OF ANTIMICROBIAL FACTOR FROM HUMAN PROMYELOCYTES IN LOW SERUM CONTAINING MEDIUM UNDER CHEMOSTAT CULTIVATION

It proves that a purifed Anti-Microbial Factor (AMF) from human promye locytes has strong activity on Gram(-) and Gram(+) bacteria, showing 0 .5 (mu g/ml) of Minimal Bacterical Concentration (MBC) on both E. coli and S. aureus. For mass production of AMF, chemostat cultivation is r ecommended to accumulate cells out of the reactor since it is an intra cellular protein and its system requires only 1% serum in the medium. Its production process proves to be closely growth-related. 1.7 x 10(- 8) (mu g/viable cell/day) of maximum specific AMF production rate is e stimated at 0.026 h(-1) of dilution rate, maintaining 6x10(6) (viable cell/ml). Ca. 300 (mg/ml) of crude AMF can be obtained for 50 days of continuous cultivation under optimal conditions. The cell growth reach es relatively fast steady state.