EFFECT OF PREPROMOTER REGION STRUCTURE ON TRANSCRIPTION BY T7 RNA-POLYMERASE

A study was made of the key reaction of isothermal nucleic acid amplif ication, namely transcription by T7 RNA polymerase. The dependence of the transcription efficiency on the length and structure of the prepro moter region was examined using model templates containing fragments o f Venezuelan equine encephalomyelitis virus DNA. Introduction of a GIC pair to the 5' end of the T7 RNA polymerase promoter ensured the maxi mal yield of the RNA transcript. Thus, the minimal length of the DNA p repromoter region was determined.