VIRD2-INDEPENDENT BUT MOBA-DEPENDENT TRANSFER OF BROAD-HOST-RANGE PLASMID RSF1010 DNA FROM AGROBACTERIUM INTO PLANT-CELL NUCLEUS

Authors
SHADENKOV AA KOVALEVA MV KUZMIN EV UZBEKOVA SV SHEMYAKIN MF
Citation
Aa. Shadenkov et al., VIRD2-INDEPENDENT BUT MOBA-DEPENDENT TRANSFER OF BROAD-HOST-RANGE PLASMID RSF1010 DNA FROM AGROBACTERIUM INTO PLANT-CELL NUCLEUS, Molecular biology, 30(2), 1996, pp. 272-275
Citations number
23
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
30
Issue
2
Year of publication
1996
Part
2
Pages
272 - 275
Database
ISI
SICI code
0026-8933(1996)30:2<272:VBMTOB>2.0.ZU;2-C
Abstract
A study was made of the efficiency of plant transformation with Agroba cterium tumefaciens strains carrying a binary vectors composed of a Ti plasmid (virD2(+) or virD(2-)) and the H22Kneo construct (oriT-mob-re taining derivative of the broad-host-range plasmid RSF1010). The pH22K neo DNA was shown to be efficiently transferred to the plant cell nucl eus even in the absence of a functional VirD2.