THE LEVEL OF A MESSENGER-RNA WITH SEQUENCE SIMILARITY TO THE OLD YELLOW ENZYME-NADPH DEHYDROGENASE INCREASES IN CHENOPODIUM-RUBRUM CELLS INRESPONSE TO CYTOKININ

Stationary-phase cells of Chenopodium rubrum were treated with t-zeati n riboside for 2 h, and the pattern of gene expression in both treated and non-treated cells was examined by differential display of mRNA. B ased on this screen, we isolated a cDNA clone whose corresponding mRNA level increased upon cytokinin treatment. Northern blot analysis conf irmed that expression of this mRNA was induced by t-zeatin riboside. T he amino acid sequence deduced from the partial cDNA showed a high lev el of similarity to the corresponding amino acid sequence of the old y ellow enzyme-NADPH dehydrogenases of fungi.