CONDITIONAL EXPRESSION OF N-MYC IN HUMAN NEUROBLASTOMA-CELLS INCREASES EXPRESSION OF ALPHA-PROTHYMOSIN AND ORNITHINE DECARBOXYLASE AND ACCELERATES PROGRESSION INTO S-PHASE EARLY AFTER MITOGENIC STIMULATION OF QUIESCENT CELLS

Citation
W. Lutz et al., CONDITIONAL EXPRESSION OF N-MYC IN HUMAN NEUROBLASTOMA-CELLS INCREASES EXPRESSION OF ALPHA-PROTHYMOSIN AND ORNITHINE DECARBOXYLASE AND ACCELERATES PROGRESSION INTO S-PHASE EARLY AFTER MITOGENIC STIMULATION OF QUIESCENT CELLS, Oncogene, 13(4), 1996, pp. 803-812
Citations number
61
Categorie Soggetti
Oncology,Biology,"Cell Biology
Journal title
ISSN journal
09509232
Volume
13
Issue
4
Year of publication
1996
Pages
803 - 812
Database
ISI
SICI code
0950-9232(1996)13:4<803:CEONIH>2.0.ZU;2-Y
Abstract
To elucidate the contribution of the N-Myc protein to neuroblastomas w e have used a synthetic inducible expression system on the basis of th e tetracycline repressor of E coli to reversibly express N-myc in a hu man neuroblastoma cell line in which expression of endogenous N-myc is barely detectable. Like the c-Myc protein, N-Myc up-regulates the exp ression of both alpha-prothymosin and ornithine decarboxylase. Inducti on of N-myc increases both the rate of DNA-synthesis and the prolifera tion rate, and shortens the G(1) phase of the cell cycle. A comparison of cell populations in which the presence of N-Myc protein was restri cted to different parts of G(0)/G(1) revealed that N-Myc is rate-limit ing for cell cycle progression during the first 5 h after serum stimul ation of quiescent cells providing direct evidence that Myc-proteins a ct early after mitogenic stimulation of quiescent cells.