GLIAL TUBES IN THE ROSTRAL MIGRATORY STREAM OF THE ADULT-RAT

Citation
P. Peretto et al., GLIAL TUBES IN THE ROSTRAL MIGRATORY STREAM OF THE ADULT-RAT, Brain research bulletin, 42(1), 1997, pp. 9-21
Citations number
49
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
03619230
Volume
42
Issue
1
Year of publication
1997
Pages
9 - 21
Database
ISI
SICI code
0361-9230(1997)42:1<9:GTITRM>2.0.ZU;2-X
Abstract
In the central nervous system cell migration is usually restricted to developmental periods and occurs mainly radially, following the radial glia. Nevertheless, in the subependymal layer of the adult rodent for ebrain tangential migration of newly generated neuronal precursors dir ected to the olfactory bulb, which follow a well-defined pathway witho ut dispersion, has been recently demonstrated. In the present study, b y using light microscopic immunocytochemistry for glia-associated anti gens (glial fibrillary acidic protein, S-100 and vimentin), and conven tional electron microscopy, we observed a dense meshwork of astrocytic cells and processes throughout the subependymal layer of the adult ra t. These cells were organized to form tangentially oriented glial tube s in the subependymal layer of the lateral ventricle and in its rostra l extension to the olfactory bulb. Glial tubes were particularly evide nt within the rostral extension and were widely intercommunicating. Us ing markers for the proliferating/migrating cells of the rostral migra tory stream (5-bromo-2'-deoxyuridine, PSA-NCAM, class III beta-tubulin ), we provide evidence that long chains of PSA-NCAM/beta-tubulin-posit ive, newly generated cells are consistently observed inside the glial tubes. These results demonstrate the existence of a peculiar glial org anization within the subependymal layer of the adult rat, consisting o f long astrocytic tubes that likely represent a new type of glial guid ance, accounting for the tangential migration of a high number of cell s along their restricted pathway, to the olfactory bulb. Copyright (C) 1997 Elsevier Science Inc.