CODING SEQUENCE AND CHROMOSOME MAPPING OF THE HUMAN GENE (CDC46) FOR REPLICATION PROTEIN HCDC46 MCM5/

We have isolated the complete cDNA sequence encoding the human homolog of the yeast replication protein Cdc46/Mcm5. The cDNA was used as a p robe for chromosomal fluorescence in situ hybridization (FISH), which localized the human Cdc46 gene (CDC46) to chromosome region 22q13.1--> q13.2. This is the fourth human Mcm gene whose chromosome region has b een determined, and it is now apparent that human Mcm genes are widely distributed in the genome. As deduced from the cDNA sequence, human p rotein hCdc46 is composed of 734 amino acids and contains a central re gion that is almost 80% identical with the yeast protein. Immunoprecip itation experiments with hCdc46-specific antibodies confirm that essen tially all nuclear hCdc46 proteins form a stable dimeric complex with protein P1Mcm3.