POINT MUTATION ANALYSIS OF ARCHIVED CYTOGENETIC SLIDE DNA

Archived Giemsa-stained cytogenetic slide repositories represent valua ble DNA resources for medical, scientific, and forensic studies. Seque ncing readily identified a Charcot-Marie-Tooth disease point mutation in a 209-bp PCR amplified product. With optimal PCR primers and amplif ication conditions, our protocol quickly and reliably isolated suffici ent DNA for at least 12 independent PCR amplification reactions for fo rensic and medical applications from single slides up to 5 years old.