G. Grafi et al., A MAIZE CDNA-ENCODING A MEMBER OF THE RETINOBLASTOMA PROTEIN FAMILY -INVOLVEMENT IN ENDOREDUPLICATION, Proceedings of the National Academy of Sciences of the United Statesof America, 93(17), 1996, pp. 8962-8967
Retinoblastoma (RB-1) is a tumor suppressor gene that encodes a 105-kD
a nuclear phosphoprotein, To date, RE genes have been isolated only fr
om metazoans. We have isolated a cDNA from maize endosperm whose predi
cted protein product (ZmRb) shows homology to the ''pocket'' A and B d
omains of the Rb protein family, We found ZmRb behaves as a pocket pro
tein based on its ability to specifically interact with oncoproteins e
ncoded by DNA tumor viruses (E7, T-Ag, E1A), ZmRb can interact in vitr
o and in vivo with the replication-associated protein, RepA, encoded b
y the wheat dwarf virus, The maize Rb-related protein undergoes change
s in level and phosphorylation state concomitant with endoreduplicatio
n, and it is phosphorylated in vitro by an S-phase kinase from endored
uplicating endosperm cells, Together, our results suggest that ZmRb is
a representative of the pocket protein family and may play a role in
cell cycle progression, Moreover, certain plant monopartite geminiviru
ses may operate similarly to mammalian DNA viruses, by targeting and i
nactivating the retinoblastoma protein, which otherwise induces G(1) a
rrest.