PREPARATIONS OF GINSENOSIDE RG(1) IMMUNOGEN AND I-125 LABELED ANTIGEN

Citation
Cb. Mei et al., PREPARATIONS OF GINSENOSIDE RG(1) IMMUNOGEN AND I-125 LABELED ANTIGEN, Journal of radioanalytical and nuclear chemistry, 206(2), 1996, pp. 201-203
Citations number
6
Categorie Soggetti
Chemistry Analytical","Nuclear Sciences & Tecnology","Chemistry Inorganic & Nuclear
ISSN journal
02365731
Volume
206
Issue
2
Year of publication
1996
Pages
201 - 203
Database
ISI
SICI code
0236-5731(1996)206:2<201:POGRIA>2.0.ZU;2-E
Abstract
This work began with the side chain of ginsenoside Rg(1) which was ace tylized with Ac2O-Pyr and oxidized by OsO4 and NaIO4 to give ginsenosi de Rg(1) aldehyde(2), which was further converted into an unsaturated ester(3) by Wittig-Horner reaction. The unsaturated ester(3) was treat ed with N2H4 and HNO2 to yield Rg(1) azide(4) which was directly conju gated with BSA to give immunogen: ginsenoside Rg(1)-BSA conjugate(5). This azide(4) was conjugated with tyramine to give ginsenoside Rg(1)-t yramine conjugate which was labelled with free (NaI)-I-125 by CH-T to yield I-125-labeled antigen: gensenoside Rg(1)-I-125-tyramine(6). The labelling rate was 40%-50% and specific activity was 3.0-4.0 MBq/mu g.