HUMAN PROSTATE-SPECIFIC ANTIGEN (HPSA) PURIFICATION AND ESTABLISHMENTOF HPSA RADIOIMMUNOASSAY

Human prostate specific antigen (hPSA) RIA was developed with hPSA and anti-PSA prepared in our laboratory. Its standard curve was linear wi th a sensitivity of 0.5 mu g/L. Serum PSA levels of 130 normal males r anged from 0 to 3.5 mu g/L (1.15 +/- 0.93 mu g/L), which are consisten t with the results of other conventional RIA. The recovery, intra- and inter-assay coefficients of variation conform to the demands of RIA, and the results of 41 samples obtained by both the PSA RIA and PSA RIA of DPC were well correlated (gamma = 0.990). PSA level of 23 patients with prostatic carcinoma was 10-400 mu g/L.