A SPONTANEOUSLY IMMORTALIZED MOUSE MICROGLIAL CELL-LINE EXPRESSING CD4

We have derived a microglial clone, named C8-B4, from the 8-day mouse cerebellum organ culture which gave rise to distinct astroglial cell l ines as previously reported. indeed. the C8-B4 clone expresses classic al microglial markers (MAC1, F4/80, 2-4G2) and appears to he derived f rom a committed microglial precursor since it does not express differe ntiation antigens present during the early stage of the monocytic line age. This microglial clone expresses two characteristics not previousl y reported for microglial cell lines: it synthesizes the CD4 molecule and produces and releases large amounts of glutamate.