DNA-DAMAGE AS A BIOMARKER OF MARINE POLLUTION - STRAND BREAKS IN SEASTARS (ASTERIAS-RUBENS) FROM THE NORTH-SEA

Citation
Jm. Everaarts et A. Sarkar, DNA-DAMAGE AS A BIOMARKER OF MARINE POLLUTION - STRAND BREAKS IN SEASTARS (ASTERIAS-RUBENS) FROM THE NORTH-SEA, Water science and technology, 34(7-8), 1996, pp. 157-162
Citations number
29
Categorie Soggetti
Water Resources","Environmental Sciences","Engineering, Civil
ISSN journal
02731223
Volume
34
Issue
7-8
Year of publication
1996
Pages
157 - 162
Database
ISI
SICI code
0273-1223(1996)34:7-8<157:DAABOM>2.0.ZU;2-9
Abstract
The DNA damage in seastars (Asterias rubens) was studied by measuring the level of integrity of DNA isolated from pyloric caeca of the organ isms in order to assess the state of pollution of the North Sea. The e ffect of various genotoxic chemicals on the integrity of DNA was deter mined by the level of double strandedness with respect to total DNA fo llowing time-dependent partial alkaline unwinding assay. On the basis of the level of integrity of DNA from seastars the study area was dist inguished into three clusters: Cluster-I, the highly polluted area (co astal and estuarine zones) with low integrity of DNA (27-43% integrity ), Cluster-II, the moderately polluted area (offshore region) with med ium integrity of DNA (49-64% integrity) and Cluster-III, less polluted area (near Dogger bank region) with high integrity of DNA (70-87% int egrity). Copyright (C) 1996 IAWQ.