MEASUREMENT OF INSULIN CONTENT AT DIFFERENT SITES OF AN INSULIN PREPARATION VIAL

Insulin sampled at various levels within several insulin preparation v ials underwent high-performance liquid chromatography and fluorescence spectrophotometry to determine insulin content, Insulin content did n ot vary with Actrapid Human Insulin(R), Monotard Human Insulin(R), and Novolin N100(R), regardless of the site of sampling, However, the ins ulin content of Novolin U40(R) tended to decrease as levels in the via l decreased, Novolin U40 was also administered subcutaneously to rabbi ts to determine its effect on blood glucose, Blood glucose levels and the frequency of convulsions due to hypoglycemia varied depending on t he site in the insulin preparation vial, Our findings suggest that var iation in insulin content at different sites of the vial may account f or unstable control of blood glucose levels in diabetic patients recei ving Novolin U40.