PRODUCTION OF IL-1-BETA AND IL-6 BY ADHERENT SPLEEN-CELLS AFTER THE STIMULATION WITH LIPOPOLYSACCHARIDES FROM SALMONELLA-ENTERITIDIS STRAIN

Three different procedures were used to isolate lipopolysaccharides fr om the Salmonella enteritidis strain 477 : phenol - water extraction w ith ethanol precipitation (LPS 1), phenol - water extraction with meth anol precipitation (LPS 2) and FPLC purification (LPS 1/1). Production of interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) was observ ed in the supernatants of adherent spleen cells of BALB/c mice after t he stimulation and cultivation of the cells. The quantity of IL-1 beta and IL-6 depended on the method of LPS isolation. The highest level o f IL-1 beta was recorded at LPS 2, and of IL-6 at the stimulation of c ells by means of LPS 1.