MONOPHENOLASE ACTIVITY OF POLYPHENOL OXIDASE FROM BLANQUILLA PEAR

Citation
Jc. Espin et al., MONOPHENOLASE ACTIVITY OF POLYPHENOL OXIDASE FROM BLANQUILLA PEAR, Phytochemistry, 44(1), 1997, pp. 17-22
Citations number
41
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00319422
Volume
44
Issue
1
Year of publication
1997
Pages
17 - 22
Database
ISI
SICI code
0031-9422(1997)44:1<17:MAOPOF>2.0.ZU;2-P
Abstract
Pear polyphenol oxidase (PPO) has been isolated by using two sequentia l phase partitionings with Triton X-114 (TX-114), The enzyme showed mo nophenolase activity when assayed on p-hydroxyphenyl propionic acid (P HPPA) with 3-methyl-2-benzothiazolinone hydrazone (MBTH) in a continuo us spectrophotometric method, with high sensitivity and precision. The initial monophenolase activity showed a lag period (tau) prior to the attainment of the steady state rate (V-ss). Both kinetic parameters, V-ss and tau, depended on the enzyme and monophenol concentrations, as well as on the presence of catalytic amounts of o-diphenol. The enzym e showed an optimum pH of 4.3 and the value of K-m toward PHPPA was 0. 5 mM. Copyright (C) 1996 Elsevier Science Ltd