GENOTYPING OF MYCOBACTERIUM-TUBERCULOSIS WITH ADDITIONAL MARKERS ENHANCES ACCURACY IN EPIDEMIOLOGIC STUDIES

Citation
R. Warren et al., GENOTYPING OF MYCOBACTERIUM-TUBERCULOSIS WITH ADDITIONAL MARKERS ENHANCES ACCURACY IN EPIDEMIOLOGIC STUDIES, Journal of clinical microbiology, 34(9), 1996, pp. 2219-2224
Citations number
22
Categorie Soggetti
Microbiology
ISSN journal
00951137
Volume
34
Issue
9
Year of publication
1996
Pages
2219 - 2224
Database
ISI
SICI code
0095-1137(1996)34:9<2219:GOMWAM>2.0.ZU;2-L
Abstract
Two highly polymorphic Mycobacterium tuberculosis genomic domains, cha racterized by hybridization to the oligonucleotide (GTG)(5), were iden tified as potential DNA fingerprinting probes. These domains were clon ed [pMTB484(1) and pMTB484(2K4), respectively] and shown to be useful for genotype analysis by Southern blotting. These probes were used to genotype geographically linked strains of M. tuberculosis previously s hown to have identical IS6110 fingerprints. Subsequent DNA fingerprint s generated dth MTB484(1) and MTB484(2K4) showed a high degree of poly morphism, allowing subclassification of IS6110-defined clusters into c omposites of smaller clusters and unique strains. Correlation of the m olecular data with patient interviews and clinical records confirmed t he sensitivity of these probes, as contacts were established only with in subclusters. These findings demonstrate the requirement for multipl e probes to accurately classify M. tuberculosis strains, even those wi th high copy numbers of IS6110. The enhanced accuracy of strain typing should, in turn, further our understanding of the epidemiology of tub erculosis.