EVALUATION OF AN INFECTIVITY STANDARD FOR REAL-TIME QUALITY-CONTROL OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 QUANTITATIVE MICROCOCULTURE ASSAYS

Authors
SCOTT WA BRAMBILLA D SIWAK E BEATTY C BREMER J COOMBS RW FARZADEGAN H FISCUS SA HAMMER SM HOLLINGER FB KHAN N RASHEED S REICHELDERFER PS
Citation
Wa. Scott et al., EVALUATION OF AN INFECTIVITY STANDARD FOR REAL-TIME QUALITY-CONTROL OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 QUANTITATIVE MICROCOCULTURE ASSAYS, Journal of clinical microbiology, 34(9), 1996, pp. 2312-2315
Citations number
32
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
34
Issue
9
Year of publication
1996
Pages
2312 - 2315
Database
ISI
SICI code
0095-1137(1996)34:9<2312:EOAISF>2.0.ZU;2-C
Abstract
Quantitative micrococulture assays of cryopreserved human immunodefici ency virus type 1-infected cell suspensions and culture supernatants w ere compared among seven assay sites. There was no significant change in titer during 1 year of storage. The overall standard deviation for infected cell suspensions was approximately 0.8 log(10) virus titer. A method for detecting deviant assay results was developed and was used to identify two donor cell preparations (n = 54) that gave consistent ly low titers.